By JON GAMBRELL

DUBAI, United Arab Emirates (AP) — Iran and the United States will hold talks Friday in Rome, their fifth round of negotiations over Tehran’s rapidly advancing nuclear program.

The talks follow previously negotiations in both Rome and in Muscat, Oman.

President Donald Trump has imposed new sanctions on Iran as part of his “maximum pressure” campaign targeting the country. He has repeatedly suggested military action against Iran remained a possibility, while emphasizing he still believed a new deal could be reached by writing a letter to Iran’s 86-year-old Supreme Leader Ayatollah Ali Khamenei to jump start these talks.

Khamenei has warned Iran would respond to any attack with an attack of its own.

Here’s what to know about the letter, Iran’s nuclear program and the tensions that have stalked relations between Tehran and Washington since the 1979 Islamic Revolution.

Why did Trump write the letter?

Trump dispatched the letter to Khamenei on March 5, then gave a television interview the next day in which he acknowledged sending it. He said: “I’ve written them a letter saying, ‘I hope you’re going to negotiate because if we have to go in militarily, it’s going to be a terrible thing.’”

Since returning to the White House, the president has been pushing for talks while ratcheting up sanctions and suggesting a military strike by Israel or the U.S. could target Iranian nuclear sites.

A previous letter from Trump during his first term drew an angry retort from the supreme leader.

But Trump’s letters to North Korean leader Kim Jong Un in his first term led to face-to-face meetings, though no deals to limit Pyongyang’s atomic bombs and a missile program capable of reaching the continental U.S.

How did previous rounds go?

Oman, a sultanate on the eastern edge of the Arabian Peninsula, has hosted the three rounds of talks between Iranian Foreign Minister Abbas Araghchi and U.S. Mideast envoy Steve Witkoff. The two men have met face to face after indirect talks, a rare occurrence due to the decades of tensions between the countries.

It hasn’t been all smooth, however. Witkoff at one point made a television appearance in which he suggested 3.67% enrichment for Iran could be something the countries could agree on. But that’s exactly the terms set by the 2015 nuclear deal struck under U.S. President Barack Obama, from which Trump unilaterally withdrew America. Witkoff, Trump and other American officials in the time since have maintained Iran can have no enrichment under any deal, something to which Tehran insists it won’t agree.

Despite that, Iran and the U.S. have held expert-level talks. Experts described that as a positive sign, though much likely remains to be agreed before reaching a tentative deal.

Why does Iran’s nuclear program worry the West?

Iran has insisted for decades that its nuclear program is peaceful. However, its officials increasingly threaten to pursue a nuclear weapon. Iran now enriches uranium to near weapons-grade levels of 60%, the only country in the world without a nuclear weapons program to do so.

Under the original 2015 nuclear deal, Iran was allowed to enrich uranium up to 3.67% purity and to maintain a uranium stockpile of 300 kilograms (661 pounds). The last report by the International Atomic Energy Agency on Iran’s program put its stockpile at 8,294.4 kilograms (18,286 pounds) as it enriches a fraction of it to 60% purity.

U.S. intelligence agencies assess that Iran has yet to begin a weapons program, but has “undertaken activities that better position it to produce a nuclear device, if it chooses to do so.”

Ali Larijani, an adviser to Iran’s supreme leader, has warned in a televised interview that his country has the capability to build nuclear weapons, but it is not pursuing it and has no problem with the IAEA’s inspections. However, he said if the U.S. or Israel were to attack Iran over the issue, the country would have no choice but to move toward nuclear weapon development.

“If you make a mistake regarding Iran’s nuclear issue, you will force Iran to take that path, because it must defend itself,” he said.

Why are relations so bad between Iran and the U.S.?

Iran was once one of the U.S.’s top allies in the Mideast under Shah Mohammad Reza Pahlavi, who purchased American military weapons and allowed CIA technicians to run secret listening posts monitoring the neighboring Soviet Union. The CIA had fomented a 1953 coup that cemented the shah’s rule.

But in January 1979, the shah, fatally ill with cancer, fled Iran as mass demonstrations swelled against his rule. The Islamic Revolution followed, led by Grand Ayatollah Ruhollah Khomeini, and created Iran’s theocratic government.

Later that year, university students overran the U.S. Embassy in Tehran, seeking the shah’s extradition and sparking the 444-day hostage crisis that saw diplomatic relations between Iran and the U.S. severed. The Iran-Iraq war of the 1980s saw the U.S. back Saddam Hussein. The “Tanker War” during that conflict saw the U.S. launch a one-day assault that crippled Iran at sea, while the U.S. later shot down an Iranian commercial airliner that the American military said it mistook for a warplane.

Iran and the U.S. have see-sawed between enmity and grudging diplomacy in the years since, with relations peaking when Tehran made the 2015 nuclear deal with world powers. But Trump unilaterally withdrew America from the accord in 2018, sparking tensions in the Mideast that persist today.

Associated Press writer Amir Vahdat in Tehran, Iran, contributed to this report.

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